Abstract
BackgroundAccurate prediction of the behaviour of oral squamous cell carcinoma (OSCC) is necessary to determine prognosis and provide appropriate treatment. Therefore, it is important to investigate potential prognostic markers to determine their predictive ability. Histological assessment of specific features at the invading front of oral squamous cell carcinomas has shown to provide accurate and reproducible prognostic information. Proliferating cell nuclear antigen (PCNA) is a nuclear marker known to reflect cell turnover and may be used as a marker for tumour aggressiveness.MethodsTwenty cases of OSCC were histologically assessed to evaluate the correlation between proliferating cell nuclear antigen expression and invasive front grading. Each case was first assessed on a haematoxylin and eosin stained slide and an invading front grading (IFG) score was determined. In order to obtain a PCNA score, immunohistological staining was carried out using the peroxidase-labelled streptavidin-biotin technique with the monoclonal antibody PC10.ResultsIn all cases, tumour islands had a periphery of intensely stained proliferating cell nuclear antigen-positive epithelial cells. The average IFG score was 8 ± 1.8, and the average PCNA score was 75% ± 11.2. Regression analysis was done using data from the IFG score and PCNA score and taking the latter as the predictor variable. The Pearson correlation coefficient was 0.134, with a p-value of 0.572.ConclusionSince the correlation between PCNA score and IFG score was not significant (p > 0.05), we conclude that there is no association between cell proliferation at the invading tumour front and the histological grading of OSCC.
Highlights
Accurate prediction of the behaviour of oral squamous cell carcinoma (OSCC) is necessary to determine prognosis and provide appropriate treatment
Each haematoxylin and eosin (H&E) section was assessed histologically for their invading front grading (IFG) scores according to the criteria outlined by Bryne [10], and matched with their existing conventional histological WHO grading [24]
Our study showed that there was an average of 75% Proliferating cell nuclear antigen (PCNA) positive cells at the invasive tumour front (ITF)
Summary
Accurate prediction of the behaviour of oral squamous cell carcinoma (OSCC) is necessary to determine prognosis and provide appropriate treatment. Histological assessment of specific features at the invading front of oral squamous cell carcinomas has shown to provide accurate and reproducible prognostic information. Despite advances in surgery and various adjunctive therapies, there is no evidence to suggest that the Accurate staging is essential to evaluate treatment protocols and provide prognostic information for patients with oral squamous cell carcinoma (OSCC). This is based on the clinical assessment of tumour size, lymph node involvement and presence of distant metastases, the TNM system. Modifications including the addition of the site of the tumour and a histopathological assessment led to the development of the
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