Assessment of platelet-rich fibrin in the maintenance and recovery of cell viability of the periodontal ligament

Lorena Bortolini Navarro,Ulisses Xavier Da Silva Neto,Fabiane Barchiki,Wilson Navarro Junior,Everdan Carneiro,Vânia Portela Ditzel Westphalen

doi:10.1038/s41598-019-55930-0

Lorena Bortolini Navarro, Ulisses Xavier Da Silva Neto + Show 4 more

Open Access

https://doi.org/10.1038/s41598-019-55930-0

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Abstract

This study analyzed the efficacy of autologous platelet-rich fibrin (PRF) in maintaining and recovering cell viability of the periodontal ligament (PDL). The PDL cells were isolated from 45 extracted teeth randomly distributed among 6 groups: 5 min, 1 h, 2 h, PRF 30 min, PRF 1 h and PRF 2 h. In the groups 5 min, 1 h and 2 h (n = 5), the teeth were kept dry in extra-alveolar times of 5 min, 1 h and 2 h respectively. The teeth of the groups PRF 30 min, PRF 1 h and PRF 2 h (n = 10) were kept dry at extra-alveolar times of 30 min, 1 and 2 h followed by immersion in PRF for 45 min. PDL cells were isolated by enzymatic digestion with type II collagenase and dispase, counted and analyzed for viability with Trypan blue vital dye in Neubauer chamber. The variables total number of cells and cell viability demonstrated that in the 5 min, 1 h and 2 h groups there was a decrease after the extra-alveolar dry times of 1 and 2 h. In comparison with the total number of cells, group 1 h, considered immediate reimplantation, did not present statistical difference when compared to the groups PRF 30 min, PRF 1 h and 2 h, a result that demonstrates that PRF assists in cell maintenance and recovery. PRF provided increased cell viability in relation to the different dry extra-alveolar times analyzed (p < 0.001). Autologous PRF presented effectiveness in maintaining and recovering PDL cells from extracted teeth and kept dry for up to 2 h.

Highlights

This study analyzed the efficacy of autologous platelet-rich fibrin (PRF) in maintaining and recovering cell viability of the periodontal ligament (PDL)
A sample of 45 teeth was analyzed, which was divided among the research groups The analyzes performed were in relation to the number of PDL cells obtained after the enzymatic dissociation and their viability (Table 1)
The lower the extra alveolar time of the avulsed tooth, the better the chances of success, the half-hour dry alveolar extra time is the gold standard for the reimplantation, until one hour the cells of the periodontal ligament are alive and the prognosis of the immediate reimplantation is adequate, the time of 2 hours was selected, because at that time the cells of the periodontal ligament are already necrotic and the reimplantation performed under these conditions leads to resorption by substitution[4]

Summary

Introduction

This study analyzed the efficacy of autologous platelet-rich fibrin (PRF) in maintaining and recovering cell viability of the periodontal ligament (PDL). PRF provided increased cell viability in relation to the different dry extra-alveolar times analyzed (p < 0.001). Autologous PRF presented effectiveness in maintaining and recovering PDL cells from extracted teeth and kept dry for up to 2 h. The media are not able to replenish the metabolites of PDL cells[7,8,9] Substances such as milk and egg white are means of storage accessible to the population and have chemical and physical properties that contribute to PDL preservation for up to 6 hours[3,10,11,12,13,14,15,16]. Hank’s balanced salt solution (HBSS) is the substance that has the highest potential in maintaining the viability of the PDL, since it has pH 7.4 and 280 Osmol/L and contains essential nutrients for the maintenance of cells, being ideal to preserve cells and tissues for the 24-hour period, but is not available[3,12,13,20,21,22]

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