Assessment of Platelet count in Normal Kashmiri population

Abstract

Platelets play a key role in both hemostasis and thrombosis. The accurate measurement of platelet count is critical for not only identifying patients with platelet dysfunction but is becoming increasingly important for the monitoring of modern antiplatelet therapy. Macrothrombocytopenia, a combination of thrombocytopenia and giant platelets, can occur in a number of diseases, including chronic immune thrombocytopenia purpura and inherited giant platelet disorders. It is present in at least 12 inherited syndromes as well as in some acquired immune-mediated and hematological disorders .We report study of our apparently Normal Kashmiri Population having variation of automated and manual platelet count which were asymptomatic without any significant bleeding history in their families. In present study, 500 samples of blood were collected from apparently Normal kashmiri Population and were processed by automated analyzer (Sysmex XT-2000i) and Manual Platelet Counting (Neubauer chamber). Multiple comparisons were done between the Automated and Manual method. The result demonstrated variation in platelet count between automated analyzer and Manual Platelet Counting.The mean value of platelet count estimated by automated analyzer was found to be 126.40 x 103/µl (Range : 47.0-394.0 x 103/µl) while the mean value of platelet count estimated by manual method was 139.06 x 103/µl (Range : 60.0-390.0 x 103/µl) and was found to be statistically significant (p<0.0001).Similarly, the mean value of platelet count estimated by automated analyzer in males and females was found to be 125.64 x 103/µl (Range : 47-394 x 103/µl) and 152.93 x 103/µl (Range : 84-223 x 103/µ) respectively. During analysis it was found that platelet count by manual method is higher as compared to the automated method in our laboratory.Key words: Platelet count,Automation, Manual, Gaint platelets.