Assessment of physiological response to an acute exercise challenge in a nutritional intervention study

Abstract

Combination of an exercise challenge model with a multi‐omics approach can be considered as a sensitive methodology to undertake a more holistic analysis of physiological responses to nutritional intervention. Few human studies have been reported which addresses the response to nutritional intervention when exposed to exercise challenge. However, it has not been reported yet how physiological response to acute exercise challenge differs from resting state when combining a multi‐omics approach. Therefore, the aim of the present study was to elucidate the mechanical response to a single bout of moderate exercise by combining omics profiling data in a nutritional intervention study. In randomized, double‐blind, placebo‐controlled study, 48 sedentary participants with body mass index (kg/m2) between 23 and 30 ingested either black raspberry (n=21) or placebo (n=24) for 4 weeks. At baseline and at the end of the study, each subject underwent an acute bout of treadmill exercise at 60% VO2max for 30 min and blood and urine samples were taken at fasting and post‐exercise state. Via next‐generation sequencing and 1H‐NMR metabolomic profiling, change in gene expression and endogenous metabolites in blood and urine was identified in response to an exercise challenge. Furthermore, integrative protein‐gene‐metabolite network would provide additional support for a deeper understanding to mechanical response to an exercise challenge test.Support or Funding InformationSupported by the Ministry of Science, ICT & Future Planning through the National Research Foundation (Bio‐synergy Research Project NRF2012M3A9C4048761).