Assessment of ova collection with or without centrifugation after ovarian slicing for In vitro fertilization of slaughterhouse specimens of Iraqi Awassi ewes

Abstract

This study aims to determine the most appropriate method for oocyte collection based on their quality and quantity by slicing with or without centrifugation and testify its ability for in vitro embryo production (IVP). The ovum was collected in the Artificial Insemination Laboratory, College of Veterinary Medicine, University of Mosul, Mosul city between 1/9/2020 to 1/11/2020. A total twenty females Genitalia slaughtered at local abattoir were collected. In vitro fertilization was done according to private lab protocols (Soran laboratory related to Soran Private Hospital for fertility disease and embryo transfer) in Irbil city/Iraq. Results of the study show no significant changes between the two methods in the extraction of Grade A oocytes. However, the slicing method, which was 33.60% of ova recovery than centrifugation ORC techniques 22.73%, ORCs method in presence Grade C oocyte higher than ORCs method which were 29.09 and 20.00%, respectively also results of in vitro fertilization show that slicing method produces embryo in high percentage 45.4% than ORCs method 25.0%. We can conclude that the best method for oocyte collection was the slicing method and this method results in the best embryo production during in vitro fertilization compared with the ORCs method.