Assessment of Nephroprotective Activity of PippalyadiAgad on Diclofenac Sodium Induced Nephrotoxicity in Albino Mice: An Experimental Study

Abstract

Background: Nephrotoxicity due to Diclofenac Sodium can be correlated with this concept of Dushi Visha and nephroprotective drugs mentioned in Ayurveda texts may play an important role in this area. Pippalyadi Agad is the herbo-mineral medication stated by Yogaratnakara for treatment of Dushi Visha. Some of the drugs in this formulation have shown efficacy in correcting nephrotoxicity. Objective: To study the nephroprotective activity of Pippalyadi Agad on diclofenac sodium induced nephrotoxicity in albino mice. Methods: The experimental study was carried out in albino mice as per CPCSEA guidelines after getting approval from the Institutional Animal Ethics Committee (IAEC). 30 albino mice, each weighing about 20-30 gm, were procured from Bharat serum and vaccines. The mice were housed in the institutional animal house in standard conditions. 30 albino mice were equally divided into five groups having six animals in each group as Trial Drug (low dose), Trial Drug (medium dose), Standard Control, Disease Control and Vehicle Control. For assessment of nephrotoxicity, the animals were monitored on Day 0, Day 15 and Day 45 by carrying out blood investigations such as BUN, Serum Creatinine, Body weight and CBC. The Data were subjected to appropriate statistical tests to derive results. Results: Pippalyadi Agad treatment at low and medium doses significantly lowered BUN and Serum Creatinine in comparison with Disease and Standard Control at Day 45. Pippalyadi Agad treatment at low and medium doses significantly (p < 0.001 and p < 0.001) increased body weights of the animals in comparison with Disease and Standard Control at Day 45. No significant difference in CBC parameters was observed in any of the Groups except the Disease Control Group on Day 15 and Day 45. Conclusion: Pippalyadi Agad has shown nephroprotective activity based on biochemical changes observed in Serum Creatinine, BUN and CBC.