Abstract
The Chicago area locks separate and control water flow between the freshwaters of Lake Michigan and the network of Illinois waterways. Under extreme storm conditions, however, the locks are opened and storm waters, untreated waste, and runoff are released directly into the lake. These combined sewer overflow (CSO) events introduce microbes, viruses, and nutrients such as nitrogen and phosphorous into nearshore waters which likely affect the native species. We collected surface water samples from four Chicago area beaches – Gillson Park, Montrose Beach, 57th Street Beach, and Calumet Beach – every two weeks from May 13 through August 5, 2014. Sampling was conducted with four biological replicates for each sampling date and location, resulting in 112 samples. Each community was surveyed through targeted sequencing of the V4 16S rRNA gene. Technical replicates were also sequenced and are included in this dataset. Taxa were identified using Mothur. Raw sequence data is available via NCBI׳s SRA database (part of BioProject PRJNA245802).
Highlights
The Chicago area locks separate and control water flow between the freshwaters of Lake Michigan and the network of Illinois waterways
Raw sequence data is available via NCBI's SRA database
Raw data is available through NCBI's BioSample database by following this link
Summary
DNA extracted from bacterial cells captured using 0.22 μm filters. Amplification of the V4 region of the 16S rRNA gene. Raw data is available through NCBI's BioSample database by following this link. BioSample IDs include: SAMN03106100, SAMN03408290, SAMN03417850, SAMN03431409, SAMN03431410, SAMN03431411, SAMN03431413, SAMN03431415, SAMN03431417, SAMN03431418, SAMN03431419, SAMN03431420, SAMN03431421, SAMN03431422, SAMN03431423, SAMN03431424, SAMN03431425, SAMN03431426, SAMN03431427, SAMN03431428, SAMN03431429, SAMN03431430, SAMN03431431, SAMN03431432, SAMN03431433, SAMN03431434, SAMN03431435, SAMN03431436, and SAMN03431437 This dataset includes microbial surveys (with replication) including an instance in which the Chicago lock system was open, releasing rain, sewage water, and runoff into the nearshore waters and disturbing the native microbial communities. The raw metagenome data is publicly available for further analysis and comparison to microbial communities within other urban and rural freshwater environments. The sampling regime provides the opportunity to consider temporal and spatial variation between microbial communities within the nearshore waters, in comparison with our laboratory's prior sequencing efforts during 2013
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