Assessment of maternal immunity to Cryptosporidium baileyi in chickens

Abstract

The degree of protection to Cryptosporidium baileyi in the progeny of infected chickens was studied. Hens at the beginning of their laying period were given orally three consecutive, large doses of C. baileyi oocysts at weekly intervals. The infection became patent after 6 days and lasted for another 6 days. Increasing serum IgG, and serum, bile, lachrymal and salivary IgA were demonstrated from their samples. These immunoglobulins were transferred to the eggs, since high levels of maternally derived IgG and lower amount of IgA were present in their yolks. Hatchlings of infected hens were divided into uninfected (UY) and infected (IY) groups, the birds in the latter receiving an oral inoculum of C. baileyi oocysts on the first day of their life. Two other groups, progeny of uninfected hens served as controls (uninfected UC, and infected IC). Maternal IgG was detected in serum samples of UY hatchlings which was eliminated by the third week. The total oocyst shedding of IY chickens was 54.3% lower than that of the controls (IC), however, the prepatent and patent periods did not show significant difference. In spite of the partial protection observed in IY birds, their humoral immune response to C. baileyi was significantly lower when compared to IC. A dot-ELISA was developed to evaluate seroconversion of infected chickens which was 100% in both infected groups. The findings of the present study suggest that infection of hens with C. baileyi results in partial protection of their progeny to this parasite, and factors other than immunoglobulins may also be transferred via the eggs.