Assessment of male sterility and antioxidant enzyme activities induced by the chemical hybridization agent SQ-1 in Foxtail Millet (Setaria italica)

Haiying Zhang,Shuqi Dong,Jixiang Wang,Yinyuan Wen,Xie Song,Yuguo Wang,Xiangyang Yuan,Pingyi Guo

doi:10.9755/ejfa.2016-10-1530

Abstract

Chemical hybridization agents (CHA) induced male sterility has long been researched in other plants, however, there is little information on the male sterility induced by CHA in foxtail millet. In this experiment, we investigated the optimized application stage and efficient dosage of the SQ-1 for inducing higher male sterility in Henggu 13 in 2015 and 2016. Some studies have suggested that CHA induced male sterility may suffer from oxidative stress. To study the physiological responses, the production and scavenging effects of the reactive oxygen species (ROS) were investigated.

Highlights

Foxtail millet (Setaria italica) is largely cultivated globally and belongs to one of the most ancient crop species that was probably domesticated in China from the wild species green foxtail nearly 11 500 years ago (Lu et al, 2009; Yang et al, 2012)
The SQ-1 treated pollen were transparent after the staining test with I2-KI (Fig. 2c), which is in contrast with the deep blue colored pollens obtained from the a b c d sterility of 95.1% was obtained at the early protogyny stage in 2015 when using a concentration of SQ-1 of 6 kg ha-1
The current study assessed the effect of SQ-1 on inducing male sterility in foxtail millet, and contributed to assessment of the relations between the metabolism of reactive oxygen species (ROS) and the SQ-1 induction of male sterility

Summary

Introduction

Foxtail millet (Setaria italica) is largely cultivated globally and belongs to one of the most ancient crop species that was probably domesticated in China from the wild species green foxtail nearly 11 500 years ago (Lu et al, 2009; Yang et al, 2012). Due to its excellent drought resistance and water management, it is produced for staple food in the arid and semiarid zones of mainly China and India before the popularity of rice and wheat arose (Bettinger et al, 2010; Zohary et al, 2012; Crawford, 2006). Heterotic vigors obtained from the expression by cross fertilization of genetically different parental lines, showed superior phenotypes to their parents, such as high yield production, increased growth and development rates, improved biomass, better quality, improved stress resistance among others (Hei et al, 2016; Kalloo et al, 2006). Many approaches have been developed to avoid self-fertilization in the case of hybrid seeds production in many cereal crops, such as emasculation, genetic male sterility (GMS), cytoplasmic male sterility (CMS), and photo-thermo-

Methods

Results

Discussion

Conclusion