ASSESSMENT OF INULA CAPPA LEAVES EXTRACTS FOR ANTIDIABETIC POTENTIAL IN EXPERIMENTAL ANIMALS

Abstract

Diabetes mellitus (DM) is a long-term disease that can be caused by a lack of insulin production by the pancreas, or by the ineffectiveness of the insulin produced. For the study, the antidiabetic effects of methanolic and 50% v/v hydroalcoholic methanolic and extracts of the leaves of Inula cappa were used as oral doses at 100, 200, and 400 mg/kg and Metformine (50 mg/kg) were administered continuously for 21 days as a single morning dose. 18 hours of fasting was used to assess the drug's anti-diabetic effects in normoglycemic rats. On the 0th, 10th, and 21st days of the experiment, glucose oxidase method readings were collected using an autoanalyzer to assess fasting blood glucose levels. Anti-oxidant parameters were determined including, total phenolic content, total flavonoid concentration, DPPH free radical scavenging activity, Hydroxyl radical scavenging activity, Scavenging of Hydrogen Peroxide, Superoxide radical scavenging assay, Nitric oxide scavenging activity and Reducing power assay. The methanolic (ICME) and hydroalcoholic (ICHE) extracts of Inula cappa leaves (100, 200, and 400 mg/kg, p.o.) passes the Oral Glucose Tolerance Test. Both extracts had a greater impact in lowering blood glucose levels. Out of which ICHE at all doses showed significant effect (P<0.05-P<0.001) when compared with control. ICME in terms of glucose tolerance at all doses had significant effects. Keywords: Diabetes mellitus, insulin, metformine, scavenging, Inula cappa.