Assessment of indigenous bacteria from biodiesel effluents contaminated site

J.O Osarumwense,F.A Igiebor

doi:10.4314/jasem.v22i2.12

Abstract

This study was conducted in order to identify indigenous microorganisms which have the capability to degrade biodiesel contaminated sites. Bacterial isolates were identified on the basis of morphological and biochemical characterization in which nine bacteria were isolated from the site, Staphylococcus aureus and micrococcus letus were found to be hydrocarbon degraders during the degradation test. The efficiency of biodegradation capability of isolates was measured by UV spectroscopy for 14 days wavelength of 600nm. The optimal temperatures at which the biodegradation occurred at 30 – 37<sup>0</sup>C. The result obtained demonstrated the potentials of these isolates in situ and/or ex situ bioremediation.Keywords: Biodiesel, bioremediation, bacteria, effluent, hydrocarbon

Highlights

Indigenous soil Microorganisms are tiny colonies of life that are found and propagated from the immediate vicinity of the growing location supplies readily available nutrients directly to the soilrhizosphere system
This study is aimed at assessing indigenous microorganisms from soil capable of degrading biodiesel effluent
Isolation of bacteria and fungi from soil sample: Bacterial species were isolated from the collected soil samples by serial dilution and agar plating method wherein the soil sample was diluted from 10-1 to 10-3 dilutions and the diluted soil samples was inoculated into a sterile Petri dish before pouring Nutrient agar plates and Potato Dextrose agar respectively

Summary

Introduction

Indigenous soil Microorganisms are tiny colonies of life that are found and propagated from the immediate vicinity of the growing location supplies readily available nutrients directly to the soilrhizosphere system. Microorganisms play an important role in making soil good for growing plants. These microorganisms can be collected and cultured. The microorganisms that have been living in the local area for a long time are best for farming because they are very powerful and effective. They have survived and can survive the extreme climatic conditions of the local environment much better than artificially produced microorganisms (Soma and Sai, 2013). Many studies have been planned to either prevent the contamination or clean up the polluted sites after the contamination (Head and Swannell, 1999; Jones, 1998; Margesin and Schinner, 1997; Sayler and Ripp, 2000). This study is aimed at assessing indigenous microorganisms (bacteria) from soil capable of degrading biodiesel effluent

Objectives

Methods

Conclusion