Assessment of in vivo and post-mortem mechanical behavior of brain tissue using magnetic resonance elastography

Abstract

The knowledge of in vivo brain tissue mechanical properties is essential in several biomedical engineering fields, such as injury biomechanics and neurosurgery simulation. Almost all existing available data have been obtained in vitro by invasive experimental protocols. However, the difference between in vivo and post-mortem mechanical properties remains poorly known, essentially due to the lack of a common method that could measure them both in vivo and ex vivo. In this study, we report the use of magnetic resonance elastography (MRE) for the non-invasive assessment of in vivo brain tissue viscoelastic properties and for the investigation of their evolution after the death. Experiments were performed on seven adult male rats. Shear storage and loss moduli were measured in vivo, just after death and at post-mortem time of ≈ 24 h . A significant increase in shear storage modulus G ′ of approximately 100% was found to occur just after death ( p = 0.002 ), whereas no significant difference was found between in vivo G ′ and G ′ at 24 h post-mortem time. No significant difference was found between shear loss modulus G ″ in vivo and just after death, whereas a decrease of about 50% was found to occur after 24 h ( p = 0.02 ). These results illustrate the ability of MRE to investigate some of the critical soft tissue biomechanics-related issues, as it can be used as a non-invasive tool for measuring soft tissue viscoelastic properties.