Assessment of In vitro wound healing potential of Hibiscus leaf extract Emulgel

Abstract

The lack of possible cure and related foible in allopathic medicines has resulted in synoptic studies in natural products as they have been discovered to be less adverse and cost effective. The identification and evaluation of therapeutic potential in medicinal products resulted to the discovery of innovative, cost-effective medicines for treatment of several illnesses such as chronic wounds. In-vitro-cell scratch testing is a cost-effective technique to understand the ability of plant extracts to treat wounds. Hibiscus rosa sinensis (Malvaceae), has been used in antifertility, as anti-inflammatory, as antiseptic and as a folk cure for the treatments of skin diseases since ancient times. Considering the above claim, an attempt was made to design emulgel formulations of the hibiscus leaf methanolic extract (HL) and their impact was compared to the marketed preparation commonly used for wound healing (WH) therapy. The emulsions were produced using various oils, such as liquid paraffin (LEG), coconut oil (CEG), olive oil (OEG) and varying Tween80 and Span80 concentrations. Using various gelling agents such as Carbopol934 in varying proportions, the emulsion was developed into emulgel. For their physicochemical parameters and percentage of drug release, all formulations were assessed. Stability studies have been carried out for three months in accordance with ICH guidelines. The % drug release of OEG was found to be 82.52% within 5 hours and produced better stability and promoted henceforth for in vitro WH studies. The cytotoxicity of OEG on L929 cells was studied by MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay and assay revealed that OEG had no cytotoxic effect and the percentage viability of L929 cells at highest concentration (200μg/mL) was observed to be 74.5. In vitro scratch assay was performed to evaluate the wound healing properties of OEG and scratch assay showed significant concentration-dependent migration and closed the gap by 24.16%, 51.66% and 86.72% in 12 hours, 24 hours and 48 hours respectively. These findings were compared to Standard (Cipladine), which showed 9.35%, 49.6% and 100% of wound closures. Our research has shown OEG's capacity to heal wounds in vitro. As a prospective source of wound healing agents, OEG of HL can therefore be suggested.