Abstract
To be effective against the oxidative damages induced by UVB irradiation in the skin, the drug needs to release from the formulation in which it was incorporated and reach the skin layers where the ROS are generated. Thus, it is very important the development of a robust and sensitive methodology to extract and quantify in different skin layers the antioxidant agent delivered from topical formulations. Therefore, in the present work suitable methods to extract and quantify quercetin in skin samples and receptor phase after in vitro penetration studies were developed. The results demonstrated that the recovery from two different layers of skin, the SC and [E+D], using two different methods of quantification (DPPH• assay and HPLC, respectively), was 93.8 % when the quercetin spiked dose was 50 µg/mL, 100.4 % when it was 100 µg/mL and 89.9 % for 250 µg/mL and the average recovery of the quercetin extraction from receptor phase when dichloromethane was used as extractor solvent was 96%. These results demonstrate that the described methods have a potential application to in vitro skin penetration studies of quercetin, since it showed to be accurate and sensitive.
Highlights
Skin is the largest human organ, and is the only organ directly exposed to ultraviolet (UV) irradiation
Recently work has demonstrated the beneficial effectiveness of topical formulations containing quercetin, a flavonoid which has well-known antioxidant activity, in inhibiting the oxidative stress and inflammation induced by UVB irradiation (Casagrande et al, 2006a)
Casagrande et al (2007) had suggested an efficient approach to evaluate the quercetin skin retention released from topical formulations, the recovery of the proposed methodology was not evaluated and to date, no study was aimed to develop a robust and sensitive method to extract and quantify quercetin from different skin layers
Summary
Skin is the largest human organ, and is the only organ directly exposed to ultraviolet (UV) irradiation. Chronic exposure to UV irradiation causes premature skin aging, considering that few tissues in the body are subjected to similar degrees of oxidative stress exposure, it has been suggested the beneficial effect of topical antioxidant, which might be a successful strategy for di-. Fonseca minishing UV radiation-mediated oxidative damage of the skin. In this context, recently work has demonstrated the beneficial effectiveness of topical formulations containing quercetin, a flavonoid which has well-known antioxidant activity, in inhibiting the oxidative stress and inflammation induced by UVB irradiation (Casagrande et al, 2006a)
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