Assessment of In-Vitro inhibitory effect of khaya tea infusion on porcine pancreatic lipase activity

Abstract

The inhibition of digestive lipases is a widely studied mechanism for the determination of the potential efficacy of natural products as anti-obesity agents. In this regard, the use of natural products might be an excellent alternative strategy for the development of safe and effective anti-obesity drugs that lack the unpleasant side effects of existing chemical drugs. The aim of this study was to assess the potential effect of Khaya tea in the treatment of obesity through the evaluation of its in vitro inhibitory effects on porcine pancreatic lipase activity. Extract was obtained by infusion of powder in water 4g/150 ml at 95°C for 10 min and was subjected to chemical analysis and kinetics studies. The in vitro inhibitory effect of Khaya tea on lipase activity was studied by assessing porcine pancreatic lipase activities at varying concentrations of khaya tea extract (0, 1, 2, 3, 4, 5 mg/ml). Results showed that Khaya tea induced a competitive inhibition (85±2%) of the porcine pancreatic lipase activity in the hydrolysis of substrate emulsified with gum arabic : I.C (inhibition Concentration)50 = 0.96 ± 0.03 mg of Khaya tea /ml. These in vitro inhibition results suggest that Khaya tea could be a potential therapeutic alternative in the treatment of obesity caused by fat-rich diets.