Assessment of in vitro Antioxidant and Antidiabetic Capacities of Medlar (Mespilus germanica)

Sebnem Selen Isbilir,Hulya Yagar,Sevilay Inal Kabala

doi:10.15835/nbha47111325

Sebnem Selen Isbilir, Hulya Yagar + Show 1 more

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https://doi.org/10.15835/nbha47111325

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Abstract

The objective of the current study was to evaluate the antioxidant activity and enzyme inhibitory effect of different parts of medlar including fruit, leaf and flower bud by using various in vitro methods, and also determination of total phenolic and flavonoid content in the samples. Ethanol extracts of medlar parts were prepared and their antioxidant activities were determined using 1,1-diphenyl-2-picryl-hydrazil (DPPH•) scavenging and β-carotene bleaching methods. The leaf extract showed the strongest antioxidant activity. DPPHradical scavenging activity was in the order of BHA > leaf > bud > fruit. This ordering was the same for β-carotene bleaching activity, tocopherol > leaf > bud > fruit. The highest total phenolic (60.3 ± 1.69 mg GAE g-1 extract) and flavonoid (14.77 ± 1.15 mg QE g-1 extract) content were determined in leaf extract. For possible antidiabetic effects of extracts, α-amylase and α-glucosidase inhibitory activities were investigated, the bud extract showed the highest inhibition activities among the all extracts.

Highlights

IntroductionKnown as the medlar, is a large shrub or small tree from Rosaceae family
Mespilus germanica, known as the medlar, is a large shrub or small tree from Rosaceae family
Nabavi et al (2011) reported that Total phenolic content (TPC) and Total flavonoid content (TFC) of methanol extracts obtained from different parts of wild medlar were in the following order: leaf > bark > fruit, which is accordance with our results

Summary

Introduction

Known as the medlar, is a large shrub or small tree from Rosaceae family. It is a typical climacteric fruit which has gained a value in human consumption and commercial importance in recent years, attracting researchers to study its chemical or nutrient compositions. The most common use of medlar fruits is raw consumption (Glew et al, 2003; Bibalani and Sayadmahaleh, 2012). Medlar regulates blood circulation, strengthens the nerves and is useful for gastric diseases in the traditional medicine (Bibalani and Sayadmahaleh, 2012). The medlar leaves are traditionally boiled to use, because local people thought of it to be good for diabetes

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