Abstract
This study evaluated the toxicity of ciprofloxacin to spleen and liver when used for the treatment of mice infected with S. typhi for seven days. The dose concentration used in these experiments was 100mg/kg. Mice were divided into two groups . The first group (negative control) was not given ciprofloxacin, but rather a sterile phosphate buffer solution (PBS) as an alternative. Ciprofloxacin was administered to the second group. After seven days , the animals were sacrificed and organs (liver and spleen) were collected . The histopathological examination showed normal hepatocytes in the liver and normal structure of spleen cells in animals of control group . However, the treated group showed dilated and congested blood vessels with perivascular inflammatory cell cuffing and acute cell swelling in the liver, as well as white pulp activation with an increased number of megakaryocyte cells in the spleen. Therefore, the current study suggests that the concentration of 100 mg/kg of ciprofloxacin is considered to be toxic to hepatocytes and splenocytes of mice during the treatment period.
Highlights
The use of antibiotics is considered as a general cause of DILI.Ciprofloxacin is a fluoroquinolones with a broad spectrum of anti-bacterial efficacy
Inhibiting topoisomerase IV interferes with the separation of the replicated chromosomal DNA in special daughter cells by cell division [3].The liver is considered to be the main organ that is responsible for the metabolism and excretion of chemotherapeutic agents, xenobiotics, and environmental pollutants; these substances, together with oxidative stress, are responsible for hepatotoxicity which is a main clinical concern [4]
The day, prior to oral inoculation, it was confirmed that all animals were pathogen-free by using bacteriological examinations which showed that the mice had a negative culture for S. typhi
Summary
The use of antibiotics is considered as a general cause of DILI (drug-induced liver injury).Ciprofloxacin is a fluoroquinolones with a broad spectrum of anti-bacterial efficacy. The day, prior to oral inoculation, it was confirmed that all animals were pathogen-free by using bacteriological examinations which showed that the mice had a negative culture for S. typhi . The mice were gavaged with 0.2ml of Salmonella typhi suspension according to the LD50 dose, while the control group was gavaged with the same amount of sterile saline [10,11].
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