Assessment of heavy metal contamination and human health risk with oxidative stress in fish (Cyprinus carpio) from Shahpur Dam, Fateh Jang, Pakistan

Abstract

The study was conducted to analyze the water quality of Shahpur Dam, Pakistan, health of native fish Cyprinus carpio, and health risks associated to humans as consumers. Metal (Pb, Cu, Cr, Fe, Ni) concentrations in water and its bioaccumulation in fish tissues (liver, gills, muscles, kidneys, and brain) were determined by using an atomic absorption spectrophotometer. Pb was abundantly found in water (8.15 mg L−1) and fish organs compared with other metals, beyond the permissible referred guidelines. Metal pollution index (MPI) was found high in fish liver followed by gill, muscle, kidney, and brain. Toxicity potential of water pollution was determined in the form of oxidative stress by analyzing catalase (CAT), glutathione S-transferase (GST), and reduced glutathione (GSH) activities in liver and gill of Cyprinus carpio, using UV spectrophotometer. The CAT activity was found higher with inhibition of 41.1% and 2.1% in liver and gill of exposed fish. Inhibition of GST in liver and gill was recorded as 29.4% and 28.1%, respectively, whereas that of the GSH was observed 12.8% and 13.9%, respectively, showing significant oxidative stress in exposed fish of the dam compared with farm fish. Consequently, people consuming the contaminated fish are prone to health impacts, which was estimated by target hazard quotient (THQ), hazard index (HI), and cancer risk (CR). No metal was found to pose potential noncarcinogenic health risk individually or collectively at present but people consuming contaminated fish regularly were expected to be at target cancer risk in 70 years or more of their lifetime.