Assessment of Growth, Physiological and Biochemical Parameters and Activities of Antioxidative Enzymes in Salinity Tolerant and Sensitive Basmati Rice Varieties

Abstract

AbstractThis investigation was undertaken to compare the level of salinity tolerance of the newly bred CSR‐30 basmati rice variety with that of the salinity sensitive HBC‐19 and Pokkali rice varieties. Twenty‐one‐day‐old hydroponically raised seedlings at 6 and 12 dS m−1 were investigated for growth, photosynthetic rate, chlorophyll content, ribulose‐1,5‐bisphosphate carboxylase‐oxygenase (Rubisco) activity, relative water content (RWC), membrane stability index (MSI), lipid peroxidation, Na/K ratio and activities and gene expression of various isoforms of antioxidative enzymes. Salinity stress led to reduction in shoot length, leaf area, dry weight, RWC, MSI, rate of photosynthesis, chlorophyll content and Rubisco activity in all the three rice varieties. The levels of reduction in these parameters were maximal in HBC‐19 followed by those in CSR‐30 and Pokkali respectively. Cumulative superoxide dismutase (SOD) activity increased in Pokkali and CSR‐30 in consonance with increase in salinity stress while it decreased in HBC‐19. The Mn‐SOD activity however, was enhanced in all three varieties in the presence of salinity stress while the activities of Fe‐SOD, Cu/Zn‐SOD and ascorbate peroxidase were decreased in HBC‐19 when compared with CSR‐30 and Pokkali. The activity of catalase (CAT) was higher in HBC‐19 when compared with its activity in CSR‐30 and Pokkali. The levels of gene expressions of the three isoforms of SOD ascertained by reverse transcriptase polymerase chain reaction were not necessarily indicative of the activities of the corresponding enzymes. Thus, despite the maximal enhancement in gene expression of Fe‐SOD in HBC‐19 in response to salinity stress, the activity of this enzyme in HBC‐19 remained low. Similarly, despite a marginal increase in gene expression of Cu‐Zn SOD in the three varieties, its activity was significantly higher in Pokkali and CSR‐30 when compared with that in HBC‐19. A significant enhancement in the activity of CAT at 12 dS m−1 in HBC‐19 when compared with CSR‐30 and Pokkali might confer a degree of tolerance to H2O2 stress in this variety in the presence of higher levels of NaCl at the seedling stage.