Assessment of Genetic Relatedness of Crossbred Chicken Populations Using Microsatellite Markers

Abstract

To measure genetic relatedness between populations, for breeding purposes, we analyzed 170 birds from six crossbred populations of three pure lines of White Leghorn chickens, using 14 microsatellite markers. All the microsatellites were polymorphic, with 2-6 alleles. The mean number of alleles per locus was 3.21. The effective number of alleles varied from 1.14 to 3.94. The observed heterozygosity varied from 0.133 to 1.00, with a mean of 0.748. The F (IS) values were mostly negative, with an average of -0.345. The mean F (ST) value was 0.056. The Nm values ranged from 1.91 to 42.17. The highest genetic identity was observed between IWI x IWK and IWK x IWI. The relation between any two groups of crosses was more than 85%. The results suggest that the crossbred populations were very closely related.