Abstract

Genetic diversity among 26 Russian and European cultivars of the common hop (Humulus lupulusL.) was studied using the ISSR–PCR technique. Twenty-one primers used provided amplification of 183 DNA fragments, 106 of which (57.9%) were found to be polymorphic. The ISSR markers, specific for some cultivars were revealed. Based on the genetic distances, cluster analysis was performed and a dendrogram was constructed, on which most of the hop cultivars formed two clusters according to their origin. Advantages of the ISSR–PCR analysis in breeding studies for classification and identification of common hop cultivars are discussed.

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