Assessment of genetic diversity in Trigonella foenum-graecum and Trigonella caerulea using ISSR and RAPD markers

Rakhee S Dangi,Prabhakar K Ranjekar,Meena D Lagu,Vidya S Gupta,Lal B Choudhary

doi:10.1186/1471-2229-4-13

Rakhee S Dangi, Prabhakar K Ranjekar + Show 3 more

Open Access

https://doi.org/10.1186/1471-2229-4-13

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Abstract

BackgroundVarious species of genus Trigonella are important from medical and culinary aspect. Among these, Trigonella foenum-graecum is commonly grown as a vegetable. This anti-diabetic herb can lower blood glucose and cholesterol levels. Another species, Trigonella caerulea is used as food in the form of young seedlings. This herb is also used in cheese making. However, little is known about the genetic variation present in these species. In this report we describe the use of ISSR and RAPD markers to study genetic diversity in both, Trigonella foenum-graecum and Trigonella caerulea.ResultsSeventeen accessions of Trigonella foenum-graecum and nine accessions of Trigonella caerulea representing various countries were analyzed using ISSR and RAPD markers. Genetic diversity parameters (average number of alleles per polymorphic locus, percent polymorphism, average heterozygosity and marker index) were calculated for ISSR, RAPD and ISSR+RAPD approaches in both the species. Dendrograms were constructed using UPGMA algorithm based on the similarity index values for both Trigonella foenum-graecum and Trigonella caerulea. The UPGMA analysis showed that plants from different geographical regions were distributed in different groups in both the species. In Trigonella foenum-graecum accessions from Pakistan and Afghanistan were grouped together in one cluster but accessions from India and Nepal were grouped together in another cluster. However, in both the species accessions from Turkey did not group together and fell in different clusters.ConclusionsBased on genetic similarity indices, higher diversity was observed in Trigonella caerulea as compared to Trigonella foenum-graecum. The genetic similarity matrices generated by ISSR and RAPD markers in both species were highly correlated (r = 0.78 at p = 0.001 for Trigonella foenum-graecum and r = 0.98 at p = 0.001 for Trigonella caerulea) indicating congruence between these two systems. Implications of these observations in the analysis of genetic diversity and in supporting the possible Center of Origin and/or Diversity for Trigonella are discussed.

Highlights

Various species of genus Trigonella are important from medical and culinary aspect
Assessment of genetic diversity in T. foenum-graecum and T. caerulea using ISSR and RAPD markers A set of 100 ISSR primers was used for initial screening of 7 accessions of T. foenum-graecum of which 40 gave amplification
In T. caerulea, of the 100 ISSR primers used for initial screening, 47 gave amplification

Summary

Introduction

Trigonella foenum-graecum is commonly grown as a vegetable. This anti-diabetic herb can lower blood glucose and cholesterol levels. Another species, Trigonella caerulea is used as food in the form of young seedlings. Trigonella caerulea is used as food in the form of young seedlings This herb is used in cheese making. The family Fabaceae includes many crops useful for food, forage, fiber, wood and ornamental purposes. In this family, a few legumes such as chickpea, soybean, fababean, fenugreek, lentil, pea etc. Legumes are capable of symbiotic nitrogen fixation enriching the soil condition suitable for a crop following the legume crop [2]

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