Abstract

Fennel (Foeniculum vulgare Mill.) is an important medicinal plant with used for various purposes in different industries. In this study 25 different ecotypes of fennel from all over Iran were collected and their genetic diversity studied by seven ISSR primers. Seven ISSR primers generated 52 amplified fragments, of which 49 were polymorphic. The highest similarity coefficient among the ecotypes was between Chahestan and Haji abad whereas the minimum similarity coefficient observed between Fozveh and Moqan. In most cases, classifications were consistent with their geographical distribution for some ecotypes (like Givi and Khalkhal in close distance) and although with similarity in climate (like Damavand and Alamot with same climate). This study revealed that ISSR marker could properly separate these ecotypes based on geographical distribution and similarity in climates and showed the wide genetic diversity among Iranian fennels. In term of conservation program, it is highly recommended at least one conservation program for several accessions with near genetical distance should be conducted.

Highlights

  • IntroductionBitter Fennel (Foeniculum vulgare Mill.) known as Raziyane in Persian is one of the oldest herbs and possesses beneficial medicinal effects, belongs to the Apiaceae family and native to Mediterranean regions (Hornok, 1992)

  • Bitter Fennel (Foeniculum vulgare Mill.) known as Raziyane in Persian is one of the oldest herbs and possesses beneficial medicinal effects, belongs to the Apiaceae family and native to Mediterranean regions (Hornok, 1992).Fennel is used for various purposes in the food, cosmetic, and medical industries

  • In this study 25 different ecotypes of fennel from all over Iran were collected and their genetic diversity studied by seven Inter simple sequence repeat (ISSR) primers

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Summary

Introduction

Bitter Fennel (Foeniculum vulgare Mill.) known as Raziyane in Persian is one of the oldest herbs and possesses beneficial medicinal effects, belongs to the Apiaceae family and native to Mediterranean regions (Hornok, 1992). Fennel has been cultivated as a medicinal and spice plant for a long time in different areas of Iran and, due to adaptation to the local environments; different genotypes show genetic diversity (Heywood, 2002). DNA-based molecular markers have been used for assessment of the genetic diversity between germplasm in many plant species. Sangwan et al, 1999; Nan et al, 2003) This method is widely applicable because there are rapid, inexpensive, require small amounts of template DNA and, unlike SSR markers, do not require prior knowledge of DNA sequences (Godwin et al, 1997). ISSR markers have been efficiently used for study of genetic diversity of different medicinal plant species and crops such as Phaseolus vulgaris L. The present work aimed to determine the efficiency of ISSR marker for the identification of genetical diversity in Iranian fennels

Plant Materials and DNA Isolation
Data Analysis
Results and Discussion
Conclusion
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