Assessment of Frozen Semen Quality and Conception Rate in Cow

Abstract

This study was conducted in Sadar upazila at Dinajpur district and Ejab Alliance Limited at Thakurgaon district of Bangladesh from March 2017 to February 2018 to investigate the frozen semen quality and conception rate in cow. Total 60 frozen semen straw were collected from six different companies (CCBDF, Ejab, AD, DAIC, Lal-Teer and BRAC) at first week of every month and subsequently up to next ten months. Straw was collected from the dealer of different companies at Upazilla Veterinary Hospital, VTH-HSTU, Dinajpur and Ejab Alliance Limited Breeding Station, Thakurgaon. Then the straw was kept in liquid nitrogen cane and transfer to the laboratory of Ejab Alliance Limited for evaluation. The frozen semen straws were thawed at 35-37˚C for 60 seconds and pooled. The post-thaw motility and morphology of spermatozoa were compared and recorded among different season, preservation time, companies and area. Post-thaw motility and number of normal spermatozoa slightly varied on preservation time. Higher motility and number of normal spermatozoa were 73±0.00 and 83±0.75 found in winter season but lowest in summer season and that was 73±0.40 and 82.2±0.47, respectively. The motility and number of normal spermatozoa of BRAC semen were 75±2.27 and 84±1.77 and the values was higher among other companies with lowest in Lal-Teer which were 72±1.61 and 79±3.52, respectively. The conception rate was statistically higher (p<0.05) in winter (65%) season, 6 months’ storage duration (66%), using BRAC semen (76.5%) and at the area of sadar, Dinajpur (62%). The conception rate was somewhat lower in summer (60%) season, 10 months of duration (62%) using Lal-Teer (53.2%) semen and at the area of Ejab Alliance Ltd. (57%) Thakurgaon.