Abstract
Several problems are being encountered in the submerged fermentation (SMF) for cellulase production, globally. In this work, in a laboratory scale operation, three different fed-batch strategies were demonstrated to attempt circumventing the problems related to a batch SMF. The processes used novel surgical waste cotton and cardboard substrate mixture and a cellulolytic fungus Trichoderma harzanium ATCC® 20846™. FPase activities of: 1.94 ± 0.05 IU/mL, 2.12 ± 0.07 IU/mL, and 1.92 ± 0.2 IU/mL were obtained for the exponential feeding strategy; pulse feeding strategy, and pH stat, respectively. For all the three fed-batch SMFs, the biomass yield coefficients (Yx/s, Yx/O2); Oxygen Uptake Rates (OUR); maintenance coefficients (mO2, ms); mass transfer co-efficients (KLa); Oxygen Transfer Rates (OTR); and the effects of viscosity, sugar accumulation, and agitation on cellulase production; were estimated. Pulse feeding strategy yielded enhancements in enzyme activities than the other two feeding strategies. Basic financial analyses have been presented.
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