ASSESSMENT OF Fe2O3 NANOPARTICLES IMPACT ON FUNCTIONAL ACTIVITY OF RATS’ PERITONEAL MACROPHAGES IN EXPERIMENTS IN VITRO AND IN VIVO

Abstract

Introduction.Today much attention is paid in the world to studying safety of nanomaterials (NM), which are synthesized and used in different spheres of human activity. Among metallic nanoparticles (NPs), iron and iron oxides are widely used in medicine and pharmacy. An important step in learning, how NPs affect living organisms, is studying their toxicity and biological activity. Macrophages are of a particular interest in this respect, which are available in various tissues and which are key cells in forming an immune response. Purpose of the study. To assess Fe 2O3 NPs influence on viability and functional activity of peritoneal macrophages in rats in experiments in vitroand in vivo. Materials and methods. Fe 2O3 NPs of 19 nm and 75 nm were obtained by chemical methods. The viability of peritoneal macrophages under the influence of Fe 2O3 NPs of 19 nm and 75 nm was defined by trypan blue and MTT test; the phagocytic activity was assessed by absorption of latex particles and cytochemically; bactericidity – by NBT-test. Results. The studies showed that incubation of macrophages with NPs in vitrocaused death of 30 % cells, whereas entering NPs into the body in vivohad no effect on their viability. NPs activated macrophage phagocytosis, formation of large phagocytic vacuoles, fagosom, lysosomes and fagolizosom as well as production of reactive oxygen forms. NPs activity depends on their size and conditions of experiments.