Abstract

Background. The current research aimed to assess the prevalence of facultative anaerobes isolated from the root canals of deciduous molars. Methods. The present research enrolled 60 children in the 6‒9-year age group based on clinical and radiographic findings. Under aseptic conditions, access cavities were prepared followed by collection of samples from infected root canals with the help of sterile paper points. The samples thus obtained were subjected for microbial assay. Results. It was found that Enterococcus faecalis was isolated in 30% of cases, Escherichia coli in 28.4%, Staphylococcus aureus in 25%, α-hemolytic Streptococci in 15% and Proteus mirabilis in 1.6% of cases. Conclusion. It was concluded from the results of the present study that the root canals of deciduous molars had predominance of facultative anaerobes, confirming its polymicrobial nature. This identification of microbes is crucial as it aids in understanding the pathogenesis of pulpal and periradicular diseases to provide effective antimicrobial irrigation and medicament for endodontic treatment.

Highlights

  • Integrity of the deciduous dentition has to be maintained until normal exfoliation for proper development and maturation of the child, growth of the facioskeletal complex to its normal occlusion along with good esthetic qualities.[1]

  • It was found that Enterococcus faecalis was isolated in 30% of cases, Escherichia coli in 28.4%, Staphylococcus aureus in 25%, α-hemolytic Streptococci in 15% and Proteus mirabilis in 1.6% of cases

  • It was concluded from the results of the present study that the root canals of deciduous molars had predominance of facultative anaerobes, confirming its polymicrobial nature

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Summary

Introduction

Integrity of the deciduous dentition has to be maintained until normal exfoliation for proper development and maturation of the child, growth of the facioskeletal complex to its normal occlusion along with good esthetic qualities.[1]. Numerous factors contribute to the development of pulp and periapical diseases, eventually creating an opportunity for the ingress of bacteria in root canals. With the advent of strict anaerobic culture techniques, their sprouted a paradigm shift in the isolation of microorganisms from aerobes to facultative and obligate anaerobes from the permanent teeth with necrotic pulp and periapical pathosis.[4,5]. Insignificant data is available regarding the identification of microbiota in deciduous teeth with pulp necrosis and periapical pathosis. Documentation exists in relation to the polymicrobial nature of microbes predominated by anaerobes in the root canals of deciduous teeth with necrotic pulp and periapical lesions similar to perma-. The current research aimed to assess the prevalence of facultative anaerobes isolated from the root canals of deciduous molars

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