Assessment of Escherichia coli O157:H7 growth in ground beef in the Greek chill chain

Abstract

A predictive mathematical model of the effect of temperature (10-47 °C) on the growth of Escherichia coli O157:H7 in natural contaminated ground beef was developed. The estimated values for the cardinal parameters Tmin, Tmax, Topt and the optimum maximum specific growth rate (μopt) of E. coli O157:H7 were found to be 3.36, 46.87, 43.16 °C and 1.385/h, respectively. The developed model was further validated against observed growth of E. coli O157:H7 in ground beef at non - isothermal chilling conditions by using two periodically changing temperature profiles with temperature ranging from 0 to 15 °C. Overall the model predicted satisfactorily the growth of E. coli O157:H7 in ground beef at dynamic temperature conditions. The model was combined with temperature data collected from ground beef chill chain in Greece in order to assess the growth of the pathogen from purchase of the product at retail to consumption. Retail storage average temperature from 50 retail cabinets in Greek super markets ranged from 0.1 to 7.4 °C with a mean of 3.2 °C and a mean standard deviation of 1.7 °C. The predicted growth of the pathogen after 7 days of storage at retail ranged between 0 and 2.03 log10 CFU/g, with an average growth to 0.31 log10 CFU/g. The growth of the pathogen during transportation from retail to domestic refrigerators ranged between 0.03 and 0.45 log10 CFU/g, with an average growth to 0.16 log10 CFU/g. The average temperature of 160 domestic refrigerators ranged from -2.7 to 18.1 °C. Differences in the temperature among the shelves of the refrigerators were observed. The predicted growth of E. coli O157:H7 in ground beef stored in domestic refrigerators for 1 day ranged between 0.00 and 2.3 log10 CFU/g. For a scenario storage of ground beef in retail for 3 days, transportation from retail domestic refrigerators over a period of 6 h and storage in domestic refrigerators for 3 days the 99th percentile of the total growth was 4.83 log10 CFU/g for storage at the upper self of the domestic refrigerator. The data and models provided in the present work can be further used in a quantitative risk assessment model of E. coli O157:H7 in ground beef consumed in Greece.