Assessment of DNA damage in obese premenopausal women with metabolic syndrome

Abstract

Background and aimObesity is a main factor in the development of metabolic syndrome (MS). Obesity and MS are associated with increased oxidative stress that might cause damage to cellular biomolecules including DNA. So, we aimed of this study to assess the DNA damage in obese women with MS. MethodsThirty obese women with MS aged 25–35years and thirty age-matched healthy non-obese women were enrolled in the study. Leukocyte DNA damage was assessed by comet assay. ResultsAmong the obese women with MS, 20% of cases met criteria for polycystic ovary syndrome (PCOS). The percent of DNA damage was significantly higher in MS patients as well as in the subgroup of MS with PCOS than in controls. A significant association was determined between the frequency of DNA damage and some anthropometric measurements. Where, body mass index (BMI), waist circumference (WC) and waist to hip ratio (WHR) showed significant association with the frequency of DNA damage. Moreover, the mean of DNA damage frequency might be influenced by the presence of combined metabolic components within MS cases. Cases with the combination of hypertension plus dyslipidemia as well as cases with the combination of hypertension plus dyslipidemia plus elevated fasting glucose showed higher levels of DNA damage than those without. ConclusionsThese findings suggest that obese women with MS have an increased risk for DNA damage. Comet assay might serve as an early marker of obesity-related complications and it might be a useful tool in disease follow-up and management. The study emphasizes significant association between DNA damage and the combined metabolic components among MS cases that might be associated with increased risk of other chronic diseases.