Assessment of Different Regeneration and Washing Solutions in an Affinity Chromatography Procedure to Purify Plantibodies

Abstract

Purification efficiency is a great challenge in plantibody production. Several regeneration + washing solutions of ProSep®-vA Ultra matrix used in plantibody HB-01 purification were assessed in this study to increase matrix stability without affecting purity and to reduce costs. In conclusion, PHB-01 purity was always higher than 90%. Citric acid (100 mM); pH 3.0/0.1% Triton X-100 + 150 mM PBS; pH 8.0 and 250 mM phosphoric acid; pH 1.5 + 150 mM PBS; pH 8.0/0.1% Triton X-100 allowed increasing purification cycles up to 60 and antibody yield 1.4 and 1.3 fold, respectively, together with a significant reduction in plantibody purification cost.