Abstract
A method for measuring daily CO 2 production and oxygen consumption of mice is described, using 55-gallon plastic drums as sealed metabolic chambers, which are vented once a day. When gas exchange and food intake measurements are performed over many consecutive days (i.e., 15 days or more), an internal calibration can be performed to take into account individual variations in nutrient absorption and food spillage, as well as production of hydrogen by intestinal fermentation. This allows daily rates of carbohydrate and fat oxidation to be established with a high degree of accuracy. The method thus allows monitoring of daily and cumulative carbohydrate and fat balances over many consecutive days. This provides a totally new type of information, inaccessible by conventional studies limited to measurements of overall energy balance, but of great potential importance for the understanding of the corrective responses which bring about body weight stability.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
