Abstract
Background and Aim: Plants play an important role in drug research, and the pharmaceutical industry is heavily reliant on natural products for new drug development. Curcumin, a natural compound contained in the rhizomes of the plant Curcuma longa Linn., has been shown to have anti-inflammatory properties in scientific studies. Cissus quadrangularis L. is a fleshy plant that can be found all over the world, especially in Asia, Africa, and a few other warm tropical areas. Boerhaavia diffusa L. (Nyctaginaceae) is present in the tropical regions of India, South America, and Africa. B. diffusa roots are commonly used in Ayurveda to treat various ailments. The aim of the study is to assess the cytotoxic, antioxidant and thrombolytic, antiinflammatory and antimicrobial properties of the aqua-alcoholic extract of the herbal mixture (Curcuma longa Linn +Cissus quadrangularis +Boerhaavia diffusa )
 Methods: Aqua-alcoholic extract of mixture of Curcuma longa Linn, Cissus quadrangularis and Boerhaavia diffusa was prepared. Phytochemical in-vitro studies were done using the CCB mixture cytotoxicity by brine shrimp lethality assay, antioxidant activity by DPPH assay, anti inflammatory activity by albumin denaturation assay, antimicrobial activity against Staphylococcus aureus, Streptococcus mutans, Escherichia coli and Candida albicans by agar well diffusion method, thrombolytic activity by clot lysis assay.
 Results: The CCB mixture showed good cytotoxic activity at varying concentration in brine shrimp lethality assay, increasing antioxidant activity with DPPH assay, increasing thrombolytic activity, increasing anti inflammatory activity with increasing concentration, the anti microbial activity was not efficient as positive controls.
 Conclusion: In this in-vitro study the CCB mixture shows improved cytotoxic, antioxidant, thrombolytic, anti-inflammatory and antimicrobial activity. Further studies on invivo animal and human clinical trials needs to be done.
Highlights
Phytochemical in-vitro studies were done using the CCB mixture cytotoxicity by brine shrimp lethality assay, antioxidant activity by DPPH assay, anti inflammatory activity by albumin denaturation assay, antimicrobial activity against Staphylococcus aureus, Streptococcus mutans, Escherichia coli and Candida albicans by agar well diffusion method, thrombolytic activity by clot lysis assay
Plants play an important role in drug production, and the pharmaceutical industry relies heavily on natural products to produce new drugs [1,2]
Our study showed significantly good antioxidant properties when compared to the standard, At 50 μl concencentration the highest reading was noted as 86.5 using DPPH assay and further analysing in wavelength of 517 nm
Summary
Plants play an important role in drug production, and the pharmaceutical industry relies heavily on natural products to produce new drugs [1,2]. A natural compound found in the rhizomes of the plant Curcuma longa Linn., has been shown in experimental studies to have antiinflammatory properties. Plants play an important role in drug research, and the pharmaceutical industry is heavily reliant on natural products for new drug development. A natural compound contained in the rhizomes of the plant Curcuma longa Linn., has been shown to have anti-inflammatory properties in scientific studies. Phytochemical in-vitro studies were done using the CCB mixture cytotoxicity by brine shrimp lethality assay, antioxidant activity by DPPH assay, anti inflammatory activity by albumin denaturation assay, antimicrobial activity against Staphylococcus aureus, Streptococcus mutans, Escherichia coli and Candida albicans by agar well diffusion method, thrombolytic activity by clot lysis assay. Conclusion: In this in-vitro study the CCB mixture shows improved cytotoxic, antioxidant, thrombolytic, anti-inflammatory and antimicrobial activity. Further studies on invivo animal and human clinical trials needs to be done
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