Assessment of cranberry bush on MCF-7 human breast cancer cells

Abstract

This research aimed to study the effects of different doses Viburnum opulus L. (VO) powder extracts at different times on human breast cancer and breast epithelial cell lines. The cytotoxic effect evalueted by using MTT method and apoptosis induction annexin V-FITC/PI staining. Comet assay was used to detect the genotoxic effect by calculating the percentage of the tail length in the DNA of both cell groups. P1 class glutathione-S-transferase (GSTP1) expression level which hasprotective feature in drug resistance metabolism was studied by immunocytochemistry method and GSTP1 and total GSTA1 metabolic protein level spectrophotometer method. This study shows that DNA damage, intracellular cytotoxicity and apoptosis have increased due to the antioxidant properties of VO powder extract.GSTP1 activities by using ethacrynic acid as substrate were found statistically significant differences between all of the groups with oneway Annova test (** P <0.01).GSTP1 overexpressed in MCF-7 control cells and expression levels decreased in the MCF-7 groups that treatment with VO powder extract at different concentrations of 25, 50 and 100 µg/µL for 24, 48 and 72 h. This study provided the information that VO powder extract may be suitable for drug therapy efficacy at the molecular level.