Abstract
Accurate and quick identification of bacterial fish disease has great importance for effective disease control and prevent economic loss in aquaculture facilities. Generally used agar media allow the growth of wide-range bacteria but new chromogenic media results in colored colonies that lead to presumptive identification without requiring biochemical or serological tests. In this study, the recently commercialized chromogenic media; chromID® CPS® Elite, chromID® CPS® Elite / Columbia CNA +5% sheep blood agar, chromID® Vibrio, chromID® MRSA Smart and chromID® Staphylococcus aureus Elite (BioMérieux, France) were tested with growth performance and colony coloration of bacterial fish pathogens. chromID® CPS® Elite and chromID® CPS® Elite / Columbia CNA +5% sheep blood agar were detected the most suitable culture media to grow for all five isolated fish pathogens (i.e., Aeromonas hydrophila, Lactococcus garvieae, Pseudomonas fluorescens, Yersinia ruckeri and Vibrio anguillarum). Of which, L. garvieae was the only one observed with green colonies. chromID® Vibrio designed for V. parahaemolyticus and V cholerae was lead dark pink/purple colonies by V. anguillarum. The chromID® MRSA Smart and chromID® S. aureus Elite were detected not effective for incubation of bacterial fish pathogens.
Highlights
Along with the fastest growing food industry, aquaculture, and the increasing production rates, it is important to identify the fish diseases accurately and quickly that these diseases can cause great economic loss during the production cycle
The system of Tanrıkul & Dinçtürk. 2021 - LimnoFish 7(2): 150-156 these chromogenic media depends on the metabolism of pathogens or enzyme substrates (Perry 2017). chromID® CPS® Elite and chromID® CPS® Elite / Columbia CNA +5% sheep blood are designed for isolation of urine specimens, Escherichia coli, Proteeae and Enterococcus; chromID® Vibrio is for presumptive identification of Vibrio parahaemolyticus and Vibrio cholerae; chromID® MRSA Smart and chromID® Staphylococcus aureus Elite for the direct screening of methicillin-resistant S. aureus (MRSA) and S. aureus in clinical microbiology
Thiosulfate Citrate Bile Sucrose (TCBS) Agar was originally designed for the isolation of V. parahaemolyticus and V. cholerae but it is used for the isolation of some Vibrio species that are infectious for fish such as V. alginolyticus, V. anguillarum, V. fischeri, V. harveyi, V. ordalii, V. splendidus and V. pelagus
Summary
Along with the fastest growing food industry, aquaculture, and the increasing production rates, it is important to identify the fish diseases accurately and quickly that these diseases can cause great economic loss during the production cycle. The use of chromogenic media engenders the inhibition of nontarget organisms with differential and selective culture characteristics and target pathogens grow as colored colonies. It is important to utilize these media for quick presumptive identification of the bacterial fish disease by easier prediagnosis in order to prevent economic loss in aquaculture facilities. Yersinia CIN agar supplies selective cultivation of Yersinia species, commonly for Y. pseudotuberculosis and Y. enterocolitica, and for isolation of infectious fish pathogen, Y. ruckeri. GSP agar is a selective agar base for detection of Pseudomonas and Aeromonas and has been using for isolation of infectious Aeromonas and Pseudomonas species in fish. The Pseudomonas selective agar base, Cetrimide agar has been especially used for the isolation of Pseudomonas aeruginosa by yellow/green colonial growth. Eosin Methylene-Blue (EMB) agar is selective for Enterobacteriaceae family, coliform bacteria and especially for E. coli by greenish metallic sheen colored colonies caused by the lactose fermentation
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