Abstract
Non-invasive oral verrucous carcinoma (OVC) and invasive well-differentiated oral squamous cell carcinoma (OSCC) have similar histopathologic findings but different biological behavior. These two malignancies must be correctly differentiated by pathologists. The aim of this study was to determine immunohistochemical (IHC) expression of Ki67 in OVC and well-differentiated OSCC. Expression of Ki67 was evaluated by IHC in 15 cases of epithelial hyperplasia with no dysplasia (A group), 15 cases of OVC (B group), 12 cases of microinvasive OSCC(C group) and 15 cases of well-differentiated OSCC (D group). There was a significant difference in Ki67 expression based on pattern distribution of immunostaining positive cells, with quantitative and semi-quantitative analyses, among the four groups ; also, between A group and each of the other three groups (P=0.0001). But there was no significant difference between B and C, C and D, and B and D groups (P>0.05). The three evaluation methods of Ki67 expression showed Ki67 (Mib-1) is not a good immunohistochemical marker to assess invasion status and differentiate OVC from well-differentiated OSCC; also, it cannot be used as a diagnostic tool to distinguish between variants of OSCC with similar grade.
Highlights
57 cases consist of epithelial hyperplasia without dysplasia (15 cases), oral verrucous carcinoma (OVC) (15 cases), microinvasive oral squamous cell carcinoma (OSCC) (12 cases) and well-differentiated OSCC (15 cases) formed samples of this study
Similar to Adegboyega et al(2005) we biopsy specimens or poorly orientated specimens, can found Ki67expression pattern may have a diagnostic cause difficulty in correct histopathologic diagnosis of non utility in distinguishing epithelial hyperplasia from invasive OVC from invasive well-differentiated OSCC carcinoma; but in contrast to the same study, we could which has a very important influence in the treatment not find its usefulness in differential diagnosis of our planning (Adegboyega et al.,2005 ; Pereira et al, 2007). malignant groups due to their overlapping patterns of in some of OVCs, anaplastic transformation Ki67 expression
Inconsistent with results of Saito et al, the present study findings demonstrated that Ki67 LI is not a good indicator to differentiate malignant tumors in our series (Saito et al, 1999).OVC and OSCC LIs ( 27.4 ± 9.11 and 63.0 ± 6.47, respectively ) were reported by Saito et al when Compared to our findings, showed a great difference in expression level of Ki67 between the two studies, in OSCC LI.( Saito et al.,1999)
Summary
Uncontrolled cell proliferation plays a critical role in the development of a wide variety of carcinomas (Gerdes et al, 1986; Mulder et al, 1992; Ramires et al, 1997) It includes a very important cellular event in oral carcinogenesis that can be evaluated by immunohistochemical (IHC) study of abnormalities in cell cycle- regulatory proteins expression (Saito et al, 1999; Adegboyega et al, 2005; Angadi and Krishnapillai, 2007). Non-invasive oral verrucous carcinoma (OVC) and invasive well-differentiated oral squamous cell carcinoma (OSCC) have similar histopathologic findings but different biological behavior. These two malignancies must be correctly differentiated by pathologists. Conclusions: The three evaluation methods of Ki67 expression showed Ki67 (Mib-1) is not a good immunohistochemical marker to assess invasion status and differentiate OVC from well-differentiated OSCC; it cannot be used as a diagnostic tool to distinguish between variants of OSCC with similar grade
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