Abstract
Periodontal diseases are chronic inflammatory diseases that are constantly associated with microorganism, conventionally scaling and root planing followed by flap surgical procedure is done to treat chronic periodontitis condition, irrigating agents are used during both sub gingival scaling and root planing procedure and in flap surgical procedure. Saline and distilled water are the commonly used irrigants and they non specifically reduce the bacterial count, Ozonised water is also used as an irrigating agent. Though it’s effect on plaque is known to be effective its effect on tissues is still questionable. The study is conducted to assess the number bacterial micro colonies formed in gingival tissues after irrigating with ozonated water and comparing it with distilled water and saline water which are the commonly used irrigating agents.
 The aim of the study is to assess the bacterial micro colonies formed in gingival tissues after irrigating with ozonated water and comparing it with distilled water and saline water which are the commonly used irrigating agents.
 A randomized, split mouth study was performed. A total of 10 patients suffering from chronic generalised periodontitis were recruited into the study; 20 gingival tissue samples were obtained from the patients at the time of surgery. Both the patient and the clinician were blinded regarding the type of the irrigating agent used. The plaque samples were transferred into carrier medium and were cultured for 24 hours; 24 h after culture, the samples were collected and total colony forming units were calculated. On counting the number of colony forming units formed, it was found that the amount of colonies formed from the samples treated with ozone showed mixed results, wherein it showed both increase and decrease in colony counts.
Highlights
Periodontal diseases are constantly associated with bacterial organisms such as Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans in subgingival environment, and gingivitis and periodontitis are the common diseases which are the affecting the tissues surrounding the tooth [1,2]
Ozonated water can be used as mouth rinse and is used to disinfect oral mucosa, and ozone water jet can be used in root canal therapy and in treating gingivitis
Considering the advantages and disadvantages of using ozone and its role in periodontics the study was designed and aimed to assess the bacterial micro colonies formed in gingival tissues after irrigating with ozonated water and comparing it with distilled water and saline water which are the commonly used irrigating agents
Summary
Periodontal diseases are constantly associated with bacterial organisms such as Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans in subgingival environment, and gingivitis and periodontitis are the common diseases which are the affecting the tissues surrounding the tooth [1,2]. Bacteria are the prime etiological agent in periodontal disease, nearly 500 bacterial agents colonise the adult mouth and these bacterial microorganisms are known to cause destructive periodontal diseases [3,4]. Mechanical removal of the biofilm by scaling and root planing followed by flap surgery along with thoroughly irrigating the surgical site and adjunctive use of antibiotics have been the conventional methods of periodontal therapy. Generator for medical use [9,10]. Medical grade ozone is a mixture of pure oxygen and pure ozone in the ratio of 0.05-5% of O3 and 9599.5% of O2. Ozonated water can be used as mouth rinse and is used to disinfect oral mucosa, and ozone water jet can be used in root canal therapy and in treating gingivitis. Ozone is used as an immunostimulant, antihypoxic, detoxicating, and antimicrobial agent
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