ASSESSMENT OF BACTERIAL CONTAMINATION LEVELS ON THE SURFACE OF THE BOVINE CARCASSES AT SLAUGHTERHOUSES OF BARISHAL CITY IN BANGLADESH

Abstract

Background: Meat industries in developing countries including Bangladesh are found to be challenged by severe hygienic and sanitation problems which are associated with heavy bacterial load on the different sites of carcasses and meat. Objectives: The major objectives of this study were to determine the bacterial load at the different risk sites of bovine carcasses and identification of pathogenic bacteria as well as to assess the associated hygienic and sanitation practices with public health significance of the isolated bacteria. Materials and Methods: The study on bacterial load of bovine carcasses was conducted on 200 swab samples of different risk sites of 20 bovine carcasses in the different slaughterhouses of Barishal City Corporation during the period from April to October 2017. The aseptically collected swab samples from different sites of bovine carcasses were processed and analyzed individually by standard bacteriological procedures for Total viable count (TVC), Total coliform count (TCC), Total Staphylococcus count (TSC) and Total Salmonella count (TSAC) and the bacterial species were identified by the conventional aerobic cultural, morphological and biochemical tests. The hygienic status and practices of slaughterhouses and workers were evaluated through a structured questionnaire survey and also a visual inspection. Results: Staphylococcus aureus (78.5%), Salmonella spp. (64.5%) and Escherichia coli (64.0%) were found widely prevalent bacteria on the surface of freshly slaughtered bovine carcasses. The mean TVC of bacteria at pre-and post-washing of the different risk sites of carcasses including neck, shoulder, rump and their used knife and workers’ hand was calculated and highest one was recorded for neck at both pre (8.17 ± 1.22) and post (8.41 ± 0.92) washings. These TVC were found higher compared to standards set by WHO. The TSC was found highest at shoulder site at both pre (7.26 ± 0.94) and post (7.43 ± 1.02) washing, whereas the neck site was found highest contaminated with E. coli (6.69 ± 1.06 and 6.87 ± 1.04) and Salmonella spp.(5.73 ± 1.05 and 6.07 ± 0.92), respectively. The questionnaire survey and visual inspection of slaughter houses revealed that none of the slaughterhouse workers received any training on slaughterhouses management and hygiene, not used any head and hair covering, protective clothes and hand gloves whereas only 25.33% workers washed their hands and 34.67% used clean water at slaughterhouses during processing of carcasses and meat. Conclusions: The slaughtering of the animals on the ground and then skinning and evisceration in the same place under poor hygienic conditions are the major risk factors for heavy bacterial contamination of carcasses which has been recognized as a threat to food safety and consumers health to foodborne illness. Therefore, it requires a serious attention from all relevant authorities to apply and maintain the basic hygienic slaughterhouse practices to prevent the bacterial contamination of carcasses to prevent food borne illness. However, these data should serve as a baseline for future comparisons in measuring the bacteriological status of beef carcasses in other districts and further research works.