Assessment of Antioxidant Activity, Total Phenolic, Total Flavonoid Content and Cytotoxic Activity of Methanol Root Extracts of Sudanese Citrus sinensis

Abstract

In the present time the medicinal plants are substantial sources of biologically active materials including phenolics and flavonoids which are a good antioxidant agent. Citrus. sinensis species are important because of their medicinal, traditional and economical uses. They are used in Sudan for treatment of various diseases traditionally. In this study the plant material was extracted with different solvents by using Soxhlet apparatus. Antioxidant activity; total phenolic; total flavonoid content; and brine shrimp lethality potential from C. sinensis methanol root extract was determined. Three fractions of the methanol extract of the root showed remarkable results in antioxidant activity, total phenolic, total flavonoid content and no genotoxic effect was observed. The total content of phenols and flavonoids of these fractions were strongly associated with the antioxidant activity. Generally, C. sinensis root can be used as source of natural antioxidant compounds, hence may also support the plant usage against diseases.