Assessment of anti-inflammatory activity of Artemisia vulgaris leaves by cotton pellet granuloma method in Wistar albino rats

Abstract

AimThe present study was made to assess the anti-inflammatory activity of methanolic extract of Artemisia vulgaris leaves by cotton pellet granuloma method. MethodThe rats weighing around 150–250 g were selected, the methanolic extracts of leaves of A. vulgaris were administered at following dose of 200 & 400 mg/kg body weight respectively after surgical insertion of cotton pellets into groin region of rats. The study was conducted as per the “Cotton Pellet Granuloma method”. A. vulgaris has shown significant anti-inflammatory activity at 200 and 400 mg/kg, whereas 400 mg/kg dose shown significant results when compared with control (P < 0.05). ResultsThe extract at the dose of 400 mg/kg had shown 55.3% inhibition in weight of wet cotton pellets and 64.06% inhibition in weight of dry cotton pellets, while the extract at the dose of 200 mg/kg had shown 33% inhibition in weight of wet cotton pellets and 20.07% inhibition in weight of dry cotton pellets, 50% inhibition of implants when compared to that of control group in “Cotton Pellet Granuloma pouch method”. ConclusionThe findings indicated the anti-inflammatory evaluation of methanolic leaf extract of A. vulgaris.