Assessment of Anthropogenic Impacts on the Genetic Diversity of Phragmites australis in Small-River Habitats

Abstract

Common reed is often used as a model plant to study the anthropogenic impacts on ecosystems at local and global scales. As a community-forming species, it is directly exposed to the impacts of human activities on the ecosystem. The aim of our study was to evaluate the patterns of genetic diversity in common reed stands located in habitats that are differently affected by anthropogenic factors. We studied whether riverbed modifications, land cover in the neighborhood of the stand and the chemical and physical parameters of the river water affect the genetic diversity of P. australis at the studied sites. Using DNA fingerprinting, we genotyped 747 plants from 42 sites located in 16 small Lithuanian rivers. Bayesian clustering and principal coordinate analysis revealed two main gene pools at the population (river) level. At the site level (i.e., considering all sites independently of their rivers), polymorphism was high even between sites in the same river. Our study revealed a negative relationship between the concentration of nitrogen compounds and the genotypic richness of P. australis populations. We did not find any correlations between the other chemical parameters of the water and the parameters of the genetic diversity. Additionally, there were no genetic differences between sites in modified and unmodified river sections or between sites that differed in land cover type in the neighborhood of the stand.