Abstract
Aim:Detection of aflatoxin B1 in Livestock compound Feed and feed ingredients by high-performance thin layer chromatography (HPTLC).Materials and Methods:Chromatography was performed on HPTLC silica gel 60 F 254, aluminum sheets by CAMAG automatic TLC sampler 4, with mobile phase condition chloroform:acetone:water (28:4:0.06). Extraction of aflatoxin B1 from samples was done as per AOAC method and screening and quantification done by HPTLC Scanner 4 under wavelength 366 nm.Results:A total of 97 livestock feed (48) and feed ingredients (49) samples received from different livestock farms and farmers were analyzed for aflatoxin B1of which 29 samples were contaminated, constituting 30%. Out of 48 livestock compound feed samples, aflatoxin B1 could be detected in 16 samples representing 33%, whereas in livestock feed ingredients out of 49 samples, 13 found positive for aflatoxin B1 representing 24.5%.Conclusion:HPTLC assures good recovery, precision, and linearity in the quantitative determination of aflatoxin B1 extracted from Livestock compound feed and feed ingredients. As more number of feed and feed ingredients are contaminated with aflatoxin B1 which causes deleterious effects in both animal and human beings, so there is a need for identifying the source of contamination, executing control measures, enabling better risk assessment techniques, and providing economic benefits.
Highlights
Mycotoxins are structurally fungal metabolites produced by Fungi, not essential to fungal growth and produced periodically under fungal stress
high-performance thin layer chromatography (HPTLC) assures good recovery, precision, and linearity in the quantitative determination of aflatoxin B1 extracted from Livestock compound feed and feed ingredients
As more number of feed and feed ingredients are contaminated with aflatoxin B1 which causes deleterious effects in both animal and human beings, so there is a need for identifying the source of contamination, executing control measures, enabling better risk assessment techniques, and providing economic benefits
Summary
Mycotoxins are structurally fungal metabolites produced by Fungi, not essential to fungal growth and produced periodically under fungal stress. They can contaminate a variety of mixed feed and food leading to animal and human health specific component species such as Aspergillus, Fusarium, and Penicilliumare able to produce these toxins that account per annum for millions of dollars in losses worldwide in condemned agriculture products [1]. Fungal metabolites generally associated with fungi belonging to the genera Alternaria, Aspergillus, Fusarium, and Penicillium. Toxigenic Alternaria and Fusarium species are often classified as field fungi, while Aspergillus and Penicillium species are considered storage fungi. The mycotoxins of interest produced by Aspergillus species include aflatoxin and ochratoxin A, while Penicillium species produces ochratoxin A, citrinin and patalin among the more important mycotoxins [2]
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