Abstract
Purpose : To assess the antiviral efficiency of β-D-glucan (BDG) on human liver cell line (WRL68) infected with dengue virus (DENV). Methods : Cytotoxic activity was assessed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Solid-phase virus binding assay was used to determine the presence of a chemical affinity between dengue virus type 2 (DENV-2) and BDG. Plaque formation assay was performed to measure the suppression of DENV-2. Results : Plaque formation assay results revealed that the inhibition of DENV infection by BDG was effective at 400 μg/mL which occurred by inhibiting virus replication. BDG inhibited DENV replication and produced minimal toxicity on WRL68 cells at 600 μg/mL in a concentration-dependent manner. Treatment of DENV-2 with the highest concentration of the BDG resulted in 60, 55, and 50 % viability at 24, 48, and 72 h, respectively. Plaque formation and binding efficiency data confirmed that BDG protected the WRL68 cells against DENV-2. Conclusion : The results indicate that in infected cells, β-D-glucan was found to be potent in inhibiting replication of the dengue virus. Keywords : Dengue, β-D-glucan, Polysaccharide, Antiviral, Plaque formation, Binding efficiency
Highlights
Dengue is a transmittable fastidious disease caused by four different types of dengue viruses (DENV1, dengue virus type 2 (DENV-2), DENV-3 and DENV-4), is spread by mosquito bite
Solid-phase virus binding assay was used to determine the presence of a chemical affinity between dengue virus type 2 (DENV-2) and BDG
Plaque formation assay results revealed that the inhibition of DENV infection by BDG was effective at 400 μg/mL which occurred by inhibiting virus replication
Summary
Dengue is a transmittable fastidious disease caused by four different types of dengue viruses (DENV1, DENV-2, DENV-3 and DENV-4), is spread by mosquito bite. DENV is an RNA virus belonging to family Flaviviridae and genus Flavivirus. DENV is transmitted by the mosquito of female Aedes. The virus is widely spread in the tropical and subtropical regions; the entire global human population is at risk [1]. Malaysia accounts for approximately 3.6 % of the fatality rate of DENV infections [2]. Most deaths resulting from DENV infections occur after the following sequence of stages: mild infection, severe haemorrhagic fever, and shock syndrome [3]
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