Abstract
The CORTCK-125 kit was evaluated and compared with the fluorimetric technique currently used in the laboratory. The radioimmunoassay is performed in antibody coated tubes using I125-labelled Cortisol as a tracer. There was good correlation between the assays (r = 0.97) on analysing patient sera but the radioimmunoassay results were higher than expected from a specific Cortisol assay. The major interfering substances are prednisone, its metabolite prednisolone and progesterone (cross reactivities of 3%, 53% and 1.4% respectively). The reproducibility of the assay was examined at three different concentrations of Cortisol on 20–24 specimens. The intra-assay CV were 20%, 11% and 13% for concentrations of 110, 370 and 920 nmol/1 respectively. Inter-assay variation over 9 assays was 17%, 17% and 19% respectively. In comparison, the within and between CV of the fluorimetric method varied between 2–7% and 6–8% respectively. The kit is convenient and simple to use; however, the precision achieved was disappointing.
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