Abstract
The main aim of the study was assessing the performance of a Protein Free Medium (PFM) in different cell culture vessels using three mouse hybridomas to produce monoclonal antibodies (mAb) specific for hepatitis B surface antigen, human alpha interferon and a human versica (proteoglycan). In parallel, the influence of the PFM on the hybridoma production kinetic patterns associated to these vessels was also studied. In conclusions, PFM allowed successful hybridoma cultures and mAb production, but showed limitations compared with serum supplemented medium in T-flask, roller-bottle, gas permeable bioreactors and hollow fiber bioreactors. As it was expected, mAb production kinetic pattern was unmodified by the PFM under assessed experimental conditions and the production kinetic pattern analysis is an important tool for indicating the best operation mode for industrial scale mAb production, but an absolute prediction cannot be totally assumed from these experiments.
Highlights
The production of monoclonal antibody could be achieved by using several culture media and technologies [1,2,3,4]
MAb production of the hybridomas was studied in Gas-Permeable Bioreactors (GPB) and Hollow Fiber Bioreactors (HFB) using TurboDomaTMHP-I as cell culture medium
According to the hybridoma performance prediction made from the production kinetic patterns, hybridomas showing Type-I or II patterns would preferentially be efficiently cultivated in discontinuous mode [15, 16]. Results obtained with these Type-I hybridomas demonstrated that the production kinetic pattern analysis is an important tool for monoclonal antibody (mAb) production prediction on large scale, but an absolute prediction cannot be totally assumed from T-flask and roller-bottle results
Summary
The production of monoclonal antibody (mAb) could be achieved by using several culture media and technologies [1,2,3,4]. Cell culture media did not guarantee a high cellular density and protein secretion, because their chemical composition was only based on saline isotonic solutions, buffering components and few nutrients [5,6]. These hitches were minimized, supplementing medium with Fetal Bovine Serum (FBS). Regulatory points to consider of the pharmaceutical industry and cost advice the adoption of serum or protein free cell culture media
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
