Assessment and degradation study of total carotenoid and ß-carotene in bitter yellow cassava (Manihot esculenta Crantz) varieties

Abstract

The assessment of the variability of total carotenoid, s-carotene, all-E, and 13 and 9-Z-s-carotene isomers in twelve bitter yellow cassava was carried out, as well its degradation in five varieties after flour processing and during storage. HPLC and UV/ visible spectrophotometry were used in sample analyses. Varieties of bitter yellow cassava presented variation in the total carotenoid contents from 1.97 - 16.33 μg/ bg and the s-carotene contents varied from 1.37 - 7.66μg/ g. The 13, 9-Zand all-E-s-carotene isomers were found in all varieties, being all-E-s-carotene the predominant one. The mean degradation among the roots after processing was of 50%. Total carotenoid complete degradation was observed with less than 30 storage days. It was also observed that the total carotenoid contents in yellow bitter cassava roots varied, probably due to the characteristics of the used varieties. Heat during processing, light and oxygen might have been the agents that most contributed to flour total carotenoids degradation. Key words: Bitter yellow cassava, s-carotene, carotenoid, flour.