Abstract
In pursuit of developing fast and accurate species-level molecular identification methods, we tested six DNA barcodes, namely ITS2, matK, rbcLa, ITS2+matK, ITS2+rbcLa, matK+rbcLa and ITS2+matK+rbcLa, for their capacity to identify frequently consumed but geographically isolated medicinal species of Fabaceae and Poaceae indigenous to the desert of Cholistan. Data were analysed by BLASTn sequence similarity, pairwise sequence divergence in TAXONDNA, and phylogenetic (neighbour-joining and maximum-likelihood trees) methods. Comparison of six barcode regions showed that ITS2 has the highest number of variable sites (209/360) for tested Fabaceae and (106/365) Poaceae species, the highest species-level identification (40%) in BLASTn procedure, distinct DNA barcoding gap, 100% correct species identification in BM and BCM functions of TAXONDNA, and clear cladding pattern with high nodal support in phylogenetic trees in both families. ITS2+matK+rbcLa followed ITS2 in its species-level identification capacity. The study was concluded with advocating the DNA barcoding as an effective tool for species identification and ITS2 as the best barcode region in identifying medicinal species of Fabaceae and Poaceae. Current research has practical implementation potential in the fields of pharmaco-vigilance, trade of medicinal plants and biodiversity conservation.
Highlights
Many species of plants belonging to multiple families are catalogued as medicinal plants on the basis of the presence of specific chemical constituents and their effects on the biological systems (Herrera et al, 2016)
Ethnobotanical investigations revealed that Crotalaria burhia has antimicrobial, anti-inflammatory, wound healing, How to cite this article Tahir et al (2018), Assessing universality of DNA barcoding in geographically isolated selected desert medicinal species of Fabaceae and Poaceae
The present study aims to re-evaluate the universality of commonly used DNA barcoding loci, ITS2, mat K, rbcLa, ITS2+mat K, ITS2+rbcLa, mat K +rbcLa, and ITS2+mat K+rbcLa by applying them on medicinal plants indigenous to harsh environment of Cholistan Desert for the first time
Summary
Many species of plants belonging to multiple families are catalogued as medicinal plants on the basis of the presence of specific chemical constituents and their effects on the biological systems (Herrera et al, 2016). Fabaceae and Poaceae are among the largest plant families having medically and therapeutically useful species all over the world (Gao et al, 2010; Dashora & Gosavi, 2013; Wariss et al, 2016). Ethnobotanical investigations revealed that Crotalaria burhia has antimicrobial, anti-inflammatory, wound healing, How to cite this article Tahir et al (2018), Assessing universality of DNA barcoding in geographically isolated selected desert medicinal species of Fabaceae and Poaceae. Have antioxidant property which is used in the treatment of infectious diseases, abdominal and spastic pain, and skin problems (Rahman et al, 2017). Cymbopogon jwarancusa is reported as expectorant and used in treatment of flu, infections and epilepsy (Ahmed et al, 2014)
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