Abstract
BackgroundComprehensive information on the effect of time and temperature storage on the measurement of elements in human, whole blood (WB) by inductively coupled plasma-dynamic reaction cell-mass spectrometry (ICP-DRC-MS) is lacking, particularly for Mn and Se. MethodsHuman WB was spiked at 3 concentration levels, dispensed, and then stored at 5 different temperatures: -70 °C, −20 °C, 4 °C, 23 °C, and 37 °C. At 3 and 5 weeks, and at 2, 4, 6, 8, 10, 12, 36 months, samples were analyzed for Pb, Cd, Mn, Se and total Hg, using ICP-DRC-MS. We used a multiple linear regression model including time and temperature as covariates to fit the data with the measurement value as the outcome. We used an equivalence test using ratios to determine if results from the test storage conditions, warmer temperature and longer time, were comparable to the reference storage condition of 3 weeks storage time at −70 °C. ResultsModel estimates for all elements in human WB samples stored in polypropylene cryovials at −70 °C were equivalent to estimates from samples stored at 37 °C for up to 2 months, 23 °C up to 10 months, and −20 °C and 4 °C for up to 36 months. Model estimates for samples stored for 3 weeks at −70 °C were equivalent to estimates from samples stored for 2 months at −20 °C, 4 °C, 23 °C and 37 °C; 10 months at −20 °C, 4 °C, and 23 °C; and 36 months at −20 °C and 4 °C. This equivalence was true for all elements and pools except for the low concentration blood pool for Cd. ConclusionsStorage temperatures of −20 °C and 4 °C are equivalent to −70 °C for stability of Cd, Mn, Pb, Se, and Hg in human whole blood for at least 36 months when blood is stored in sealed polypropylene vials. Increasing the sample storage temperature from −70 °C to −20 °C or 4 °C can lead to large energy savings. The best analytical results are obtained when storage time at higher temperature conditions (e.g. 23 °C and 37 °C) is minimized because recovery of Se and Hg is reduced. Blood samples stored in polypropylene cryovials also lose volume over time and develop clots at higher temperature conditions (e.g., 23 °C and 37 °C), making them unacceptable for elemental testing after 10 months and 2 months, respectively.
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