Assessing the Influence of Dyes Physico-Chemical Properties on Incorporation and Release Kinetics in Silk Fibroin Matrices.

Bruno Thorihara Tomoda,Fabiana Perrechil,Murilo Santos Pacheco,Juliane Viganó,Mariana Agostini De Moraes,Yasmin Broso Abranches

doi:10.3390/polym13050798

Bruno Thorihara Tomoda, Fabiana Perrechil + Show 4 more

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https://doi.org/10.3390/polym13050798

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Journal: Polymers	Publication Date: Mar 5, 2021
Citations: 4	License type: CC BY 4.0

Affiliation: Universidade Federal de São Paulo

Abstract

Silk fibroin (SF) is a promising and versatile biodegradable protein for biomedical applications. This study aimed to develop a prolonged release device by incorporating SF microparticles containing dyes into SF hydrogels. The influence of dyes on incorporation and release kinetics in SF based devices were evaluated regarding their hydrophilicity, molar mass, and cationic/anionic character. Hydrophobic and cationic dyes presented high encapsulation efficiency, probably related to electrostatic and hydrophobic interactions with SF. The addition of SF microparticles in SF hydrogels was an effective method to prolong the release, increasing the release time by 10-fold.

Highlights

Silk is a natural protein produced in the form of fibers by insect larvae, such as silkworms, spiders, scorpions, mites, and flies, varying in amino acid composition, chemical structure and properties according to its origin [1,2,3]
We proceeded with the comparison between rhodamine B (RhB) and neutral red (NR) to assess the influence of the charge; methylene blue (MB) (319.85 g/mol) and rose bengal (RB) (973.67 g/mol) to assess the effect of molar mass; and MB and RhB to assess the hydrophilicity influence
The results show an increase of 15% to 65% in the silk fibroin (SF) microparticles mean diameter when the dyes were incorporated

Summary

Introduction

Silk is a natural protein produced in the form of fibers by insect larvae, such as silkworms, spiders, scorpions, mites, and flies, varying in amino acid composition, chemical structure and properties according to its origin [1,2,3]. Several studies were carried in the production of silk fibroin (SF) based materials, showing different processes for the incorporation of active compounds, as well as the effects on the delivery system, highlighting the versatility of this protein [2,4,5,6]. The “heavy” chain is mainly formed by ordered hydrophobic macromolecules and “light” chain is formed by polar amino acid residues. Due to these different blocks, SF is capable to perform hydrophilic and hydrophobic interactions, the latter being the main interaction of SF structure [8]. SF has mild processing conditions and can be used to produce hydrogels, microparticles or films [6,9]

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