Assessing the Impact of Proline and Abscisic Acid on Salt Stress Grown BRASSICA NAPUS at Germination and Seedling Establishment

Abstract

Abiotic stress such as Salinity causes serious challenge to plant growth and development, so it causes negative influence on plant's growth and yield. Different growth regulators and osmolytes kept attention as productive strategy and help the plant for enhancing stress tolerance and plant' growth and yield improved under stress condition. This project investigates the performance of Brassica napus at germination and seedling stage after application of abscisic acid and proline with combination of salt stress. Experiment was designed in completely randomized manner and petri plates were arranged in different sets with 10 seeds/each and applied with different concentrations of NaCl solutions. Proline (10 -20mM) and ABA (50-100mg/L) was pplied separately and in combination (proline 10 x ABA 50) and (proline 20 x 100) in these sets. After 8 days experiment was terminated and different parameters (germination percentage, seedling growth, shoot and root length, fresh and dry biomass, vigor index, Reletive water content, (RWC) Water content (WC), Root Shoot Ratio (RSR), Shoot Weight Ratio (SWR) and Root Weight Rratio (RWR). Salt stress exhibited significant decrease in shoot and root length, fresh and dry biomass, vigor index, RWC, different ratios (SWR, RWR) and different physiological indices while RSR exhibited increase in comparison to control in all sets. According to this research the application of proline and abscisic acid alone showed increased performance in all studied parameters. Interactions of proline and ABA did not support germination and seedling establishment process. This study concluded that application of proline (10mM) and ABA (100mg/L) improved germination and seedling establishment process very well as compared to other doses in Brasicca napus