Assessing the effects of experimental bacterial challenge with Pasteurella multocida and ampicillin on the respiratory microbiota of pre-weaned Holstein calves

Abstract

The association between changes in the respiratory microbiota and Bovine Respiratory Disease (BRD) in dairy calves is not well understood. We investigated characteristics of the nasopharyngeal (NP) microbiota associated with BRD following Pasteurella multocida infection. We also evaluated the effect of ampicillin on the respiratory microbiota. Calves (n = 30) were inoculated with P. multocida and randomly allocated into an antibiotic group (AMP; n = 17) or placebo group (PLAC; n = 11) when lung lesions developed. Deep NP swabs (DNPS) were collected before and after challenge. Monitoring was performed daily until euthanasia at day 14. Swabs and tissue samples were collected for analysis. The V4 hypervariable region of the 16 S rRNA gene was amplified and sequenced on an Illumina MiSeq. Increased species abundance in the pre-challenge DNPS was associated with a decrease in cumulative respiratory disease over 14 days post-infection. While NP beta diversity was affected by infection, antibiotic therapy showed no effect on the alpha and beta diversity nor the relative abundance (RA) of genera in the NP tonsil, lymph node and lung microbiota. Antibiotic therapy was associated with an increased RA of NP Pasteurella spp. and a decreased RA of NP Prevotella spp. Common taxa among all samples included GIT-associated bacteria, which suggests a possible link between the GIT microbiota and respiratory microbiota in dairy calves.